[Abstract]

Objective: To investigate the anti-inflammation effect and possible mechanism of Salvianic acid A (SAA) in mouse peritoneal macrophages.Methods: Peritoneal macrophages which were obtained from BALB/c mice, were randomly assigned to five groups bellow (n=3 in each):negative control; LPS group, receiving 10μg/ml LPS; SAA pretreated groups, three concentrations of SAA (10μg/ml, 1μg/ml, 0.1μg/ml) + 10μg/ml LPS. Nitric oxide (NO) was determined by Griess method, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in supernatant were determined by Radio-immunity, matrix metalloproteinase-9 (MMP-9) was measured by Gelatin zymography, protein expressions of inducible nitric oxide synthase (iNOS) and activation of nuclear factor-kappa B (NF-κB) in the extract were measured by Western Blot.Results: The levels of NO, TNF-α, IL-6, iNOS and MMP-9 increased rapidly after stimulated by 10μg/ml LPS (P<0.01), all of which were reduced by pretreated with SAA in a concentration-dependent manner (P<0.01). LPS stimulation led to the NF-κB P65 translocation from cytosol to the nucleus sharply, but this effect is significantly being suppressed by SAA at concentration of 10μg/ml.Conclusion: The results showed that SAA had strong anti- inflammatory effects in LPS-stimulated peritoneal macrophages. The important mechanism is due to its inhibition of NF-κB activation.

Title: Salvianic Acid A Inhibits Induction of Inflammatory Mediators by Blocking Nuclear Factor-κB Activation in Macrophages

Category: Tumor Biol

Filename: Salvianic Acid A Inhibits Induction of Inflammatory Mediators by Blocking Nuclear Factor-κB Activation in Macrophages.pdf

Pages: 163

Price: US$48.00

Buy This Paper:

Download:

Donated for this research subject:

FAVORITE: ADD TO FAVORITE

Contact: E-Mail:medpaper@hotmail.com

                TEL:1-888-774-999A

How to get this paper's electronic documents?

1, Click the "Buy Now" button to complete the online payment

2, Download the paper's electronic document from the successful payment return page/Or the system will send this paper's electronic document to your E-Mail within 24 hours

Version:zh-cn

Perhaps You will be interested in these papers

• 2012-05-16 The Effect of Specific Nuclear Factor Kapaa-B p65 Short Interference RNA Interference on the Proliferation and Apotosis of THP-1 Cell Lines

• 2012-05-14 Study for Triptolide in Protection Against Lps-induced Acute Lung Injury in Rats

• 2012-05-11 The Effect and Mechanism of Recombinant Human Endostatin in Proliferation and the Capacity of Invasion of Cervical Cancer Hela Cells

• 2012-03-13 The Protective Effect of Ulinastatin on Acute Lung Injury and Its Mechanism in Mice

• 2012-03-04 The Study of the Alterations of Some Inflammatiory Mediators and the Protective Effect and Possible Mechanism of Edaravone in the Lipopolysaccharide-induced Acu

• 2012-03-02 The Correlations between the Expressions of PTEN and MMP-9 and the Clinical Biological Behavior in Gastric Carcinoma and Regional Lymph Node

• 2012-02-23 The Effect of Prenatal Exposure to Lipopolysaccharide on Intra-uterine Fetal Growth Development and Skeletal Development in Mice

• 2012-02-18 Preparation of a Liposomal Lipopolysaccharide of Gram-Negative Bacteria Isolated from Clinic and Its Immunoprotective Effects

• 2012-01-29 Role of KLF4 in Regulating the Expression of IL-6 Induced by LPS

• 2012-01-28 The Clinical Value of Serum IL-8 and MMP-9 Detection in the Patients of Non-small Cell Lung Cancer